1,4 - ß cellobiosidase (CbsA) in Xanthomonas bacteria involved in switch to non vascular infection phenotype shows large scale deletions and structural changes

AUTHOR(S)

Varshini A, Shreya S, Kruthika T R, Shilpa S K, Keerthi B, Manohar G M

DOI: https://doi.org/10.46647/ijetms.2023.v07i03.128

ABSTRACT
Vascular plant pathogens spread through the veins of the host causing systemic infections whereas non vascular pathogens are confined to infection sites and cause localized symptom development. CbsA -1,4 beta cellobiosidase is a cell wall-degrading enzyme responsible for causing vascular infection and a mutant version of it is found in non vascular infection. Here, we investigate the sequence variation and structural changes accompanying a mutant CbsA gene present in some pathovars exhibiting non vascuar infection by Xanthomonas bacteria and also chalk out the evolutionary history of the non vascular phenotype. Among X.oryaze pv oryaze and X. oryzae pv oryzicola , X.oryzicola showed a number of large scale deletions and amino acid substitutions. Protein structure of X. oryaze pv oryaze showed 12 helices and X. oryzae pv oryzicola showed missing 2 helices. X. citri pv vignicola did not show the same large scale deletions however the sequence had accumulated large variation. The non vascular phenotype may have evolved in the ancestor of X. citri, X.oryzae and X. oryzicola pathovars, but may have reverted back in X. oryzae. We elaborate that sequence and structural changes accompanying just one gene might have had a major role in the phenotypic swift from vascular to nonvascular infection. This may have implications in plant disease because vascular infecting pathogens are efficient in invading the whole body of the plant while non vascuar infection is localized.

Page No: 832 - 839

References:

1. Redkar, Amey, Mugdha Sabale, and Antonio Di Pietro. "A'Hydrolase Switch'for Vascular Specialization in Plant Pathogenic Bacteria." Trends in Plant Science 26.5 (2021): 427-429.
2. Costa, Joana, et al. "Integrating Science on Xanthomonas and Xylella for Integrated Plant Disease Management." Microorganisms 11.1 (2022): 6.
3. Ryan, Robert P., et al. "Pathogenomics of Xanthomonas: understanding bacterium–plant interactions." Nature Reviews Microbiology 9.5 (2011): 344-355.
4. Gluck-Thaler, Emile, et al. "Repeated gain and loss of a single gene modulates the evolution of vascular plant pathogen lifestyles." Science advances 6.46 (2020): eabc4516.
5. Cao, Jianbo, et al. "Different cell wall-degradation ability leads to tissue-specificity between Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola." Pathogens 9.3 (2020): 187.
6. Roman-Reyna, Verónica, et al. "Genome resource of barley bacterial blight and leaf streak pathogen Xanthomonas translucens pv. translucens strain UPB886." Plant disease 104.1 (2020): 13-15.
7. Mew, T. W. "Xanthomonas oryzae pathovars on rice: cause of bacterial blight and bacterial leaf streak." Xanthomonas. New York: Chapman and Hall (1993): 30-40.
8. Dharmapuri, Sridhar, and Ramesh V. Sonti. "A transposon insertion in the gumG homologue of Xanthomonas oryzae pv. oryzae causes loss of extracellular polysaccharide production and virulence." FEMS microbiology letters 179.1 (1999): 53-59.
9. Tayi, Lavanya, et al. "A mutation in an exoglucanase of Xanthomonas oryzae pv. oryzae, which confers an endo mode of activity, affects bacterial virulence, but not the induction of immune responses, in rice." Molecular plant pathology 19.6 (2018): 1364-1376.
10. He, Ya-Wen, et al. "Rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae produces multiple DSF-family signals in regulation of virulence factor production." BMC microbiology 10 (2010): 1-9.
11. Stothard, Paul. "The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences." Biotechniques 28.6 (2000): 1102-1104.
12. Tamura, Koichiro, Glen Stecher, and Sudhir Kumar. "MEGA11: molecular evolutionary genetics analysis version 11." Molecular biology and evolution 38.7 (2021): 3022-3027.
13. Paysan-Lafosse, Typhaine, et al. "InterPro in 2022." Nucleic Acids Research 51.D1 (2023): D418-D427.
14. Mirdita, Milot, et al. "ColabFold: making protein folding accessible to all." Nature methods 19.6 (2022): 679-682.
15. Jumper, John, et al. "Highly accurate protein structure prediction with AlphaFold." Nature 596.7873 (2021): 583-589.




How to Cite This Article:
Varshini A, Shreya S, Kruthika T R, Shilpa S K, Keerthi B, Manohar G M .1,4 - ß cellobiosidase (CbsA) in Xanthomonas bacteria involved in switch to non vascular infection phenotype shows large scale deletions and structural changes . ijetms;7(3):832-839. DOI: 10.46647/ijetms.2023.v07i03.128