International Journal of Engineering Technology and Management Sciences

2023, Volume 7 Issue 2

Screening and production of bacterial amylase from different Strepomyces species

AUTHOR(S)

Dr. Rachana Choudhary, Dr. Bhuneshwari Nayak

DOI: https://doi.org/10.46647/ijetms.2023.v07i02.093

ABSTRACT
The enzymes from the microbial sources are more stable and can be obtained from cheap sources. Amylase is most important industrial enzyme which is widely used in food and biofuel industries. Streptomyces is a group of bacteria belongs to largest genus of Actinobacteria and commonly isolated from the soil. Streptomyces species have been widely used as microbial cell in industries for production of antibiotics. For the production of antibiotics, Streptomyces species are frequently used as microbial cells. This study reported that screening and production of amylase from different Streptomyces species i.e. Streptomyces microflavus, Streptomyces cyaneus and Streptomyces diastaticus. The cultures were screened for the production of amylase by starch agar plate assay. Result shown the Streptomyces diastaticus was better amylase enzyme producer compare to Streptomyces microflavus and Streptomyces cyaneus. Streptomyces diastaticus gives maximum starch hydrolysis by showing clear zone (4.4 mm), enzyme production (1.60 U/ml) and protein concentration (2.25 mg/ml).

Page No: 841 - 844

References:

Acharyabhatta, A., Kandula, S.K. &Terli, R. (2013). Taxonomy and polyphasic characterization of alkaline amylase producing marine Streptomyces rochei BTSS 1001. International Journal of Microbiology, 1-8. DOI:10.1155/2013/276921
Al-Dhabi, N.A., Esmail, G.A., Ghilan, A.M., Arasu, M.V., Duraipandiyan, V. &Ponmurugan, K. (2020). Isolation and purification of starch hydrolyzing amylase from Streptomyces sp. Al-Dhabi-46 obtained from the Jazan region of Soudi Arabia with industrial applications. Journal of King Saud University – Science, 32, 1226-1232.
Bull, A.T., Stach, J.E.M., Ward, A.C. &Goodfellow, M. (2005). Marine actinobacteria; perspectives, challenges, future directions. Antonie Van Leeuwehoek, 87, 65-79.
Chakraborty, S., Raut, G., Khopade, A., Mahadik, K. &Kokare, C. (2012). Study on calcium ion dependent amylase from haloalkaliphilic marine Streptomyces strain A3. Indian Journal of Biotechnology, 11, 427-37.
Hamilton, L.M., Kelly, C.T. & Fogarty, W.M. (1999). Purification and properties of them raw starch-degrading ɑ-amylase of Bacillus sp. IMD 434. Biotechnology Letters, 21, 111-115.
Islam, M.S., Aktar, M.B. & Rahman, M.M. (2014). Determination of alpha-amylase activity of Streptomyces spp. isolated from Bangladeshi soils. International Journal of Interdisciplinary studies, 1, 167-170.
Li, H.F., Chi, Z.M., Wang, X.H. & Ma, C.L. (2007). Amylase production by the marine yeast Aureobasidium pullulans N13d. Journal of Ocean University of China, 6, 60-65. DOI: 10.1007/s11802-007-0060-3
Pandey, A., Nigam, P., Soccol, C.R., Soccol, V.T. & Sing, D. (2000). Advances in microbial amylases. Biotechnology and Applied Biochemistry, 31, 135-152. DOI: 10.1042/BA19990073
Prakash, O. & Jaiswal, N. (2010). ɑ-Amylase: An ideal representative of thermostable enzymes. Applied Biochemistry and Biotechnology, 160, 2401-2414. DOI: 10.1007/s12010-009-8735-4
Rengasamy, S. & Thangaprakasam, U. (2018). Isolation, screening and determination of a-amylase activity from marine Streptomyces species. International Journal of Pharmacy and Pharmaceutical Sciences, 10, 122-127.
Sanghvi, G.V., Koyani, R.D. & Rajput, K.S.(2011). Isolation, optimization, and partialpurification of amylase from Chrysosporium asperatum by submerged fermentation. Journal of Microbiology and Biotechnology,21, 470–476.
Sreejetha, M., Divya, Veena, S. & KokatiVenkata, B.R. (2016). The bioactive potential of Streptomyces variabilis-DV-35 isolated from Thottada Marine sediments, Kannur, Kerala. Asian Journal of Pharmaceutical and Clinical Research, 9, 67-71.
Takahashi, Y. &Omura, S. (2003). Isolation of new actinomycete strains for the screening of new bioactive compounds. The Journal of General and Applied Microbiology, 45, 141-156.
Tonkova, A. (2006). Microbial starch converting enzymes of the a-amylase family. Microbial Biotechnology in Horticulture, 1, 421–472.
Vidyalakshmi, R., Paranthaman, R. & Indhumathi, J. (2009). Amylase production on submerged fermentation by Bacillus spp. World Journal of Chemistry, 4, 89–91.
Vigal, T., Gil, J.F., Daza, A., Garcia-Gonzalez, M.D. & Martin, J.F. (1991). Cloning characterization and expression of an alpha-amylase gene from Streptomyces griseus IMRU 3570. Molecular Genetics and Genomics, 225, 278-88.


How to Cite This Article:
Dr. Rachana Choudhary, Dr. Bhuneshwari Nayak . Screening and production of bacterial amylase from different Strepomyces species . ijetms;7(2):841-844. DOI: 10.46647/ijetms.2023.v07i02.093