International Journal of Engineering Technology and Management Sciences

2023, Volume 7 Issue 3

Antioxidant, Antibacterial Activities and GC-MS Analysis of Methanol Leaves Extract of Barleria prionitis L

AUTHOR(S)

Pooja K

DOI: https://doi.org/10.46647/ijetms.2023.v07i03.107

ABSTRACT
Barleria prionitis is an important medicinal plant that belongs to the Acanthaceae family. It was used for various medicinal purposes in Ayurvedic medicine and herbal remedy. The juice of the leaves is applied to the feet to prevent cracking in the monsoon season. This paper is used to evaluate the antioxidant and antibacterial activities of methanol extract of leaves of B. prionitis and Gas Chromatography-Mass Spectrometry (GC-MS) analysis for active compound identification. Radical scavenging assay and reduction assay methods are used for antioxidant activity and the well diffusion method is used for evaluating antibacterial activity. The heat-induced hemolysis assay method is used for the antihemolytic activity. HP-5 column is used for GCMS analysis. The IC50 of DPPH radical scavenging activity of methanol leaves extract of B. prionitis is 221.68 µg/mL concentration, the IC50 of ABTS●+ radical cation scavenging activity is 4.28 µg/mL concentration and the IC50 of superoxide radical scavenging activity is 86.88 µg/mL concentration. The RC50 of phosphomolybdenum reduction of methanol leaves extract of B. prionitis was 44.31 µg/mL concentration and the RC50 of Fe3+ reduction is 151.88 µg/mL concentration. The IC50 of antihemolytic activity is 91.98 µg/mL concentration. The antibacterial activity showed a maximum zone of inhibition of 20 mm for Staphylococcus aureus at 500 µg/mL concentration. Antioxidant compounds such as flavone, 2H-1-Benzopyran-4,7-diol,3,4-dihydro-2-phenyl, and 6methoxyflavone as well as antimicrobial compound c-terpinen are evaluated by GC-MS analysis.

Page No: 695 - 708

References:

    1. Amoo, S.O., Finnie, J.F. and Van Staden, J. "In vitro pharmacological evaluation of three Barleria species." Journal of Ethnopharmacology, 121(2), pp.274-277,2009.
    2. Rajinder, K., Gurdeep, K., Sandhya, G., Amit, K. and Taminder, K. "Preliminary phytochemical screening and in vitro anthelmintic activity of whole plant extracts of Barleria prionitis Linn. against earth worms: Pheretima posthuma." World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS), 4(7), pp.1340-1347,2015.
    3. Khare, D. and Tiwari, K.L. "Effect of leaf exudates and leaf tissue sap of Barleria prionitis L, on spore germination of some spices of anamorphic fungi." Int J Res Eng Appl Sci, 6(4), pp.1-7,2016.
    4. Banerjee, D., Maji, A.K., Mahapatra, S. and Banerji, P. "Barleria prionitis Linn.: a review of its traditional uses, phytochemistry, pharmacology and toxicity." Research journal of phytochemistry, 6(2), pp.31-41,2012.
    5. Hajjaj, G., Bahlouli, A., Sayah, K., Tajani, M., Cherrah, Y. and Zellou, A. "Phytochemical screening and in-vivo antipyretic activity of the aqueous extracts of three Moroccan medicinal Plants." Pharm. Biol. Eval, 4, pp.88-92,2017.
    6. Gangula, A., Podila, R., Karanam, L., Janardhana, C. and Rao, A.M. "Catalytic reduction of 4-nitrophenol using biogenic gold and silver nanoparticles derived from Breynia rhamnoides" Langmuir, 27(24), pp.15268-15274,2011.
    7. Spanos, G.A. and Wrolstad, R.E. "Influence of processing and storage on the phenolic composition of Thompson seedless grape juice." Journal of agricultural and food chemistry, 38(7), pp.1565-1571,1990.
    8. Liu, X., Dong, M., Chen, X., Jiang, M., Lv, X. and Yan, G. "Antioxidant activity and phenolics of an endophytic Xylaria sp. from Ginkgo biloba." Food chemistry, 105(2), pp.548-554,2007.
    9. Blois, M.S., "Antioxidant determinations by the use of a stable free radical." Nature, 181, pp.1199-1200,1958.
    10. Ravishankara, M.N., Shrivastava, N., Padh, H. and Rajani, M., "Evaluation of antioxidant properties of root bark of Hemidesmus indicus R. Br.(Anantmul)." Phytomedicine, 9(2), pp.153-160,2002.
    11. Delgado-Andrade, C., Rufián-Henares, J.A. and Morales, F.J. "Assessing the antioxidant activity of melanoidins from coffee brews by different antioxidant methods." Journal of Agricultural and Food Chemistry, 53(20), pp.7832-7836,2002.
    12. Sharmila, K.J., Monisha, S.M., Beevi, A.A. and Deebarathi, V. "Antibacterial, antioxidant, anticancer effects and GCMS analysis of Berberis aristata”. Biomedicine, 40(3), pp.286-293,2020.
    13. Yen, G.C. and Chen, H.Y. "Antioxidant activity of various tea extracts in relation to their antimutagenicity."Journal of agricultural and food chemistry, 43(1), pp.27-32,1995.
    14. Yang, Z.G., Sun, H.X. and Fang, W.H. "Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice."Vaccine, 23(44), pp.5196-5203,2005.
    15. Kubo, I., Muroi, H. and Himejima, M."Antimicrobial activity of green tea flavor components and their combination effects." Journal of Agricultural and Food Chemistry, 40(2), pp.245-248,1992.
    16. Harini, V., Vijayalakshmi, M., Sivaraj, C. and Arumugam, P. "Antioxidant and anticancer activities of methanol extract of melochia corchorifolia l." Int. J. of Sci. and Res, 6(1), pp.1310-1316,2017. 
    17. Primiano, T., Sutter, T.R. and Kensler, T.W."Redox regulation of genes that protect against carcinogens." Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 118(3), pp.487-497,1997.
    18. Middleton, Jr., MD, E."Biological properties of plant flavonoids: an overview." International Journal of Pharmacognosy, 34(5), pp.344-348,1997.
    19. Kedare, S.B. and Singh, R.P. "Genesis and development of DPPH method of antioxidant assay. " Journal of food science and technology, 48, pp.412-422,2011.
    20. Gülçin, İ., Oktay, M., Küfrevioğlu, Ö.İ. and Aslan, A."Determination of antioxidant activity of lichen Cetraria islandica (L) Ach."Journal of ethnopharmacology, 79(3), pp.325-329,2002
    21. Hagerman, A.E., Riedl, K.M., Jones, G.A., Sovik, K.N., Ritchard, N.T., Hartzfeld, P.W. and Riechel, T.L. "High molecular weight plant polyphenolics (tannins) as biological antioxidants. " Journal of agricultural and food chemistry, 46(5), pp.1887-1892,1998.
    22. Loo, A.Y., Jain, K. and Darah, I."Antioxidant activity of compounds isolated from the pyroligneous acid, Rhizophora apiculata." Food chemistry, 107(3), pp.1151-1160,2008.
    23. Siddhuraju, P. and Becker, K. "The antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata (L.) Walp.) seed extracts."Food chemistry, 101(1), pp.10-19,2007.
    24. Amarowicz, R., Pegg, R.B., Rahimi-Moghaddam, P., Barl, B. and Weil, J.A. "Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. " Food chemistry, 84(4), pp.551-562,2004.
    25. Alam, N., Yoon, K.N., Lee, J.S., Cho, H.J. and Lee, T.S. "Consequence of the antioxidant activities and tyrosinase inhibitory effects of various extracts from the fruiting bodies of Pleurotus ferulae." Saudi journal of biological sciences, 19(1), pp.111-118,2012.
    26. Bhandary, S.K., Bhat, V.S., Sharmila, K.P. and Bekal, M.P."Preliminary phytochemical screening of various extracts of Punica granatum peel, whole fruit and seeds." Journal of Health and Allied Sciences NU, 2(04), pp.34-38,2012.

How to Cite This Article:
Pooja K .Antioxidant, Antibacterial Activities and GC-MS Analysis of Methanol Leaves Extract of Barleria prionitis L . ijetms;7(3):695-708. DOI: 10.46647/ijetms.2023.v07i03.107

Copyright © 2017-18,IJETMSJOURNAL. All Rights reserved.